Motion Analysis of Live Objects by Super-Resolution Fluorescence Microscopy

Motion analysis plays an important role in studing activities or behaviors of live objects in medicine, biotechnology, chemistry, physics, spectroscopy, nanotechnology, enzymology, and biological engineering. This paper briefly reviews the developments in this area mostly in the recent three years, especially for cellular analysis in fluorescence microscopy. The topic has received much attention with the increasing demands in biomedical applications. The tasks of motion analysis include detection and tracking of objects, as well as analysis of motion behavior, living activity, events, motion statistics, and so forth. In the last decades, hundreds of papers have been published in this research topic. They cover a wide area, such as investigation of cell, cancer, virus, sperm, microbe, karyogram, and so forth. These contributions are summarized in this review. Developed methods and practical examples are also introduced. The review is useful to people in the related field for easy referral of the state of the art

Locomotion capabilities of a modular robot with eight pitch-yaw-connecting modules

This is an electronic version of the paper presented at the International Conference on Climbing and Walking Robots, held in 2006 on BrusselsIn this paper, a general classification of the modular robots is proposed, based on their topology and the type of connection between the modules. The loco- motion capabilities of the sub-group of pitch-yaw con- necting robots are analyzed. Five different gaits have been implemented and tested on a real robot composed of eight modules. One of them, rotating, has not been previously achieved. All gaits are implemented using a simple and elegant central pattern generator (CPG) ap- proach that simplify the algorithms of the controlling system

An Uncertainty-aware Hybrid Approach for Sea State Estimation Using Ship Motion Responses

Situation awareness is essential for autonomous ships. One key aspect is to estimate the sea state in a real-time manner. Considering the ship as a large wave buoy, the sea state can be estimated from motion responses without extra sensors installed. This task is challenging since the relationship between the wave and the ship motion is hard to model. Existing methods include a wave buoyanalogy (WBA) method, which assumes linearity between wave and ship motion, and a machine learning (ML) approach. Since the data collected from a vessel in the real world is typically limited to a small range of sea states, the ML method might suffer from catastrophic failure when the encountered sea state is not in the training dataset. This paper proposes a hybrid approach that combined the two methods above. The ML method is compensated by the WBA method based on the uncertainty of estimation results and, thus, the catastrophic failure can be avoided. Real-world historical data from the Research Vessel (RV) Gunnerus are applied to validate the approach. Results show that the hybrid approach improves estimation accuracy.acceptedVersio

Data-driven sea state estimation for vessels using multi-domain features from motion responses

Situation awareness is of great importance for autonomous ships. One key aspect is to estimate the sea state in a real-time manner. Considering the ship as a large wave buoy, the sea state can be estimated from motion responses without extra sensors installed. However, it is difficult to associate waves with ship motion through an explicit model since the hydrodynamic effect is hard to model. In this paper, a data-driven model is developed to estimate the sea state based on ship motion data. The ship motion response is analyzed through statistical, temporal, spectral, and wavelet analysis. Features from multi-domain are constructed and an ensemble machine learning model is established. Real-world data is collected from a research vessel operating on the west coast of Norway. Through the validation with the real-world data, the model shows promising performance in terms of significant wave height and peak period.acceptedVersio